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Paroxysmal Nocturnal Hemoglobinuria phenotype

Alias/Synonym: PNH, Flow Cytometry

CPT Code: 88184, 88185x7, 88187

Includes:
Glycophorin A, CD59 RBC, Flaer, CD64, CD45, CD14, CD15, CD24
Specimen Type, Preferred:
Peripheral blood
Specimen Type, Alternate:
Sample Quantity, Preferred:
5-10mL
Sample Quantity, Minimum:
1mL whole blood
Transport Container:
Green top (Sodium Heparin) tube
Lavender top (EDTA) tube
Transport Temperature:
Whole Blood: Room temperature
Collection Instructions:
Please submit a copy of the CBC if available. Transfusion history is required.
If testing will occur in less than eight hours, specimens must be handled and transported in a manner to assist in the maintenance of the specimen between 18-25 degrees Celsius prior to testing.
If testing is expected to occur in greater than eight hours, specimens must be handled and transported with cold packs to maintain specimens between 2 and 8 degrees Celsius prior to testing.
In event of a dry tap (no aspirate for flow cytometry) submit 5-10mL of whole blood (Sodium Heparin) or a separate 10mm core biopsy in sterile RPMI.
Specimen should be received within 24 hours of collection for optimal cell viability.
Specimen Stability:
Room temperature: 2 day(s)
Refrigerated: 2 day(s)
Frozen: Unacceptable
Rejection Criteria:
Bone marrow sample
Clotted
Frozen
Lithium tube.
Hemolyzed
Clinical Significance:
Immunophenotypic analysis of PNH Cells
Limitations:
Fresh Specimen
Turnaround Time:
1 day(s)
Reference Ranges:
NA
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