Paroxysmal Nocturnal Hemoglobinuria phenotype

Alias/Synonym: PNH, Flow Cytometry

CPT Code: 88184, 88185x7, 88187

Includes:

Glycophorin A, CD59 RBC, Flaer, CD64, CD45, CD14, CD15, CD24

Specimen Type, Preferred:

Peripheral blood

Specimen Type, Alternate:

Sample Quantity, Preferred:

5-10mL

Sample Quantity, Minimum:

1mL whole blood

Transport Container:

Green top (Sodium Heparin) tube

Lavender top (EDTA) tube

Transport Temperature:

Whole Blood: Room temperature

Collection Instructions:

Please submit a copy of the CBC if available. Transfusion history is required.

If testing will occur in less than eight hours, specimens must be handled and transported in a manner to assist in the maintenance of the specimen between 18-25 degrees Celsius prior to testing.

If testing is expected to occur in greater than eight hours, specimens must be handled and transported with cold packs to maintain specimens between 2 and 8 degrees Celsius prior to testing.

Specimen should be received within 24 hours of collection for optimal cell viability.

Specimen Stability:

Room temperature: 2 day(s)

Refrigerated: 2 day(s)

Frozen: Unacceptable

Rejection Criteria:

Bone marrow sample

Clotted

Frozen

Lithium tube.

Hemolyzed

Clinical Significance:

Immunophenotypic analysis of PNH Cells

Limitations:

Fresh Specimen

Turnaround Time:

1 day(s)

Reference Ranges:

NA

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