Alias/Synonym: Tumor Suppressor Gene Mutation

CPT Code: 88342

Specimen Type, Preferred:
Formalin fixed paraffin embedded tissue
Specimen Type, Alternate:
Sample Quantity, Preferred:
1 block
2 slides
Sample Quantity, Minimum:
Transport Container:
Paraffin block
Unstained slides
Transport Temperature:
Room temperature
Collection Instructions:
Block and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Slides should be positively charged and cut at 3-4 microns for each stain/antibody ordered.
Specimen Stability:
Rejection Criteria:
No liquid specimens.
Uncharged slides
Clinical Significance:
P53 is a tumor suppressor gene located on the short arm of chromosome 17. It is normally expressed in all nucleated cells, but has a very short half-life and it not normally detected by immunohistochemistry. It accumulates during cell injury and arrests the cell cycle so repair can take place. Mutated P53 shows impaired DNA-binding, allowing increased genomic instability. It has a prolonged half-life, lending itself to detection by IHC. It is implicated in a wide variety of tumor types, although some tumors in particular can show very strong P53 expression (e.g. ovarian serous carcinoma).
Turnaround Time:
1 day(s)
Reference Ranges: