p53
Alias/Synonym: Tumor Suppressor Gene Mutation
CPT Code: 88342
- Includes:
- Specimen Type, Preferred:
- Formalin fixed paraffin embedded tissue
- Specimen Type, Alternate:
- Sample Quantity, Preferred:
- 1 block
- 2 slides
- Sample Quantity, Minimum:
- N/A
- Transport Container:
- Paraffin block
- Unstained slides
- Transport Temperature:
- Room temperature
- Collection Instructions:
- Block and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
- Slides should be positively charged and cut at 3-4 microns for each stain/antibody ordered.
- Specimen Stability:
- N/A
- Rejection Criteria:
- No liquid specimens.
- Uncharged slides
- Clinical Significance:
- P53 is a tumor suppressor gene located on the short arm of chromosome 17. It is normally expressed in all nucleated cells, but has a very short half-life and it not normally detected by immunohistochemistry. It accumulates during cell injury and arrests the cell cycle so repair can take place. Mutated P53 shows impaired DNA-binding, allowing increased genomic instability. It has a prolonged half-life, lending itself to detection by IHC. It is implicated in a wide variety of tumor types, although some tumors in particular can show very strong P53 expression (e.g. ovarian serous carcinoma).
- Limitations:
- Turnaround Time:
- 1 day(s)
- Reference Ranges:
- N/A