NextGen Sequencing HotSpot Cancer Panel
Alias/Synonym: NGS Hotspot
CPT Code: 81450
- Includes:
- ABL1, AKT1, ALK, APC, ATM, BRAF, CDH1, CDKN2A, CSF1R, CTNNB1, EGFR, ERBB2, ERBB4, EZH2, FBXW7, FGFR1, FGFR2, FGFR3, FLT3, GNA11, GNAQ, GNAS, HNF1A, HRAS, IDH1, IDH2, JAK2, JAK3, KDR, KIT, KRAS, MET, MLH1, MPL, NOTCH1, NPM1, NRAS, PDGFRA, PIK3CA, PTEN, PTPN11, RB1, RET, SMAD4, SMARCB1, SMO, SRC, STK11, TP53, VHL
- Specimen Type, Preferred:
- Formalin fixed paraffin embedded tissue
- Specimen Type, Alternate:
- Sample Quantity, Preferred:
- 1 Block
- 8 Slides
- Sample Quantity, Minimum:
- 1 Block
- 5 Slides
- Transport Container:
- Paraffin block
- Unstained slides
- Transport Temperature:
- Room temperature
- Collection Instructions:
- Charged slides should be cut at 4-5 microns thick.
- Specimen Stability:
- Room temperature: Indefinite
- Rejection Criteria:
- Frozen
- Clinical Significance:
- Comprehensive test that detects common hotspot mutations related to numerous cancer types.
- Limitations:
- This test is designed to detect gene mutations only. It is not intended to detect copy number changes or gene rearrangements.
- Turnaround Time:
- 10-14 day(s)
- Reference Ranges:
- Cutoff value % based on assay sensitivity