KRAS Mutation Analysis
Alias/Synonym:
CPT Code: 81275, 81276
- Includes:
- Codons 12, 13, 61, 117, 146
- Specimen Type, Preferred:
- Bone marrow aspirate
- Formalin fixed paraffin embedded tissue
- Specimen Type, Alternate:
- Peripheral blood
- Sample Quantity, Preferred:
- 2mL aspirate
- 10 mL peripheral blood
- 1 Block
- Sample Quantity, Minimum:
- 0.5mL aspirate
- 5mL peripheral blood
- 5 unstained slides
- Transport Container:
- Lavender top (EDTA) tube
- Green top (Sodium Heparin) tube
- Paraffin block
- Unstained slides
- Transport Temperature:
- Refrigerated for tubes
- Room temperature for blocks
- Collection Instructions:
- If bone marrow is dry tap, submit 5-10mL peripheral blood.
- Specimen Stability:
- Room temperature: 2 day(s)
- Refrigerated: 3 day(s)
- Rejection Criteria:
- Room temperature or clotted for tubes
- Frozen
- Clinical Significance:
- Detects mutations in exons 2, 3 and 4 (codons 12, 13, 61, 117 and 146) of the KRAS gene.
- Limitations:
- This test is designed to detect gene mutations only. It is not intended to detect copy number changes or gene rearrangements.
- Turnaround Time:
- 7 - 10 day(s)
- Reference Ranges:
- Cutoff value % based on assay sensitivity