KRAS Mutation Analysis


CPT Code: 81275, 81276

Codons 12, 13, 61, 117, 146
Specimen Type, Preferred:
Bone marrow aspirate
Formalin fixed paraffin embedded tissue
Specimen Type, Alternate:
Peripheral blood
Sample Quantity, Preferred:
2mL aspirate
10 mL peripheral blood
1 Block
Sample Quantity, Minimum:
0.5mL aspirate
5mL peripheral blood
5 unstained slides
Transport Container:
Lavender top (EDTA) tube
Green top (Sodium Heparin) tube
Paraffin block
Unstained slides
Transport Temperature:
Refrigerated for tubes
Room temperature for blocks
Collection Instructions:
If bone marrow is dry tap, submit 5-10mL peripheral blood.
Specimen Stability:
Room temperature: 2 day(s)
Refrigerated: 3 day(s)
Rejection Criteria:
Room temperature or clotted for tubes
Clinical Significance:
Detects mutations in exons 2, 3 and 4 (codons 12, 13, 61, 117 and 146) of the KRAS gene.
This test is designed to detect gene mutations only. It is not intended to detect copy number changes or gene rearrangements.
Turnaround Time:
7 - 10 day(s)
Reference Ranges:
Cutoff value % based on assay sensitivity