EGFR Mutation Analysis – Cell Free DNA in Plasma
Alias/Synonym: EGFR, cfDNA, Liquid Biopsy, ctDNA, T790M
CPT Code: 81235
- Includes:
- Specimen Type, Preferred:
- Plasma, derived from Peripheral Blood
- Specimen Type, Alternate:
- Peripheral Blood
- Sample Quantity, Preferred:
- 2mL Plasma
- 10 mL Peripheral blood
- Sample Quantity, Minimum:
- 2mL Plasma
- 5mL Peripheral blood
- Transport Container:
- Plasma – Sterile container
- Blood - Lavender top (EDTA) tube
- Transport Temperature:
- Plasma – Frozen (-20 to -25 C)
- Peripheral blood: Refrigerated (2-8 C)
- Collection Instructions:
- Plasma separated from blood within four hours of blood draw. Blood must have been fixed in K2EDTA Tube
- Specimen Stability:
- Frozen Plasma – Up to 12 months
- Refrigerated blood 0 Up to 48 hrs
- Rejection Criteria:
- Plasma – Unfrozen
- Peripheral blood -
- Clotted
- Hemolyzed
- Clinical Significance:
- This test is designed to assess acquired resistance mutations, specifically T790M, to EGFR tyrosine kinase inhibitors in recurrent lung cancer patients.
- Limitations:
- Turnaround Time:
- 7 - 10 day(s)
- Reference Ranges:
- Quantitative analysis : cutoff value %