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Zap-70 Analysis in CLL

Alias/Synonym: Zap 70

CPT Code: 88184, 88185x5, 88187

Includes:
cZAP-70, CD19, CD3, CD56, CD5, CD45
Specimen Type, Preferred:
Bone marrow
Peripheral blood
Specimen Type, Alternate:
Fresh tissue
Cerebral spinal fluid
Fine needle aspirate
Pleural fluid
Ascites fluid
Sample Quantity, Preferred:
2-3 mL bone marrow
5-10mL whole blood
0.5cmx0.5cmx0.25cm 1 piece of tissue
10-100mL of fresh fluid
Sample Quantity, Minimum:
0.5mL bone marrow
1mL whole blood
100mg fresh tissue
3mL fresh fluid
(minimum volume is dependent on cellularity)
Transport Container:
Green top (Sodium Heparin) tube
Lavender top (EDTA) tube
RPMI Medium
Sterile saline
Transport Temperature:
Bone marrow/Whole Blood: Room temperature
Tissue/fluid: Refrigerated
Collection Instructions:
Please submit a copy of the CBC if available.
If testing will occur in less than eight hours, specimens must be handled and transported in a manner to assist in the maintenance of the specimen between 18-25 degrees Celsius prior to testing.
If testing is expected to occur in greater than eight hours, specimens must be handled and transported with cold packs to maintain specimens between 2 and 8 degrees Celsius prior to testing.
In event of a dry tap (no aspirate for flow cytometry) submit 5-10mL of whole blood (Sodium Heparin) or a separate 10mm core biopsy in sterile RPMI.
Specimen should be received within 24 hours of collection for optimal cell viability.
Specimen Stability:
Room temperature: 2 day(s)
Refrigerated: 2 day(s)
Frozen: Unacceptable
Rejection Criteria:
Clotted
Frozen
Lithium tube
Hemolyzed
Any form of fixatives
Clinical Significance:
Analysis of Zap 70 expression for CLL prognosis
Limitations:
Fresh Specimen Prior/current diagnosis of CLL is required.
Turnaround Time:
1 day(s)
Reference Ranges:
NA
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