Plasma Cell Phenotype
Alias/Synonym:
CPT Code: 88184, 88185x31, 88189
- Includes:
- Kappa, Lambda, CD10, CD5, CD200, CD34, CD38, CD20, CD19, TCRgamma delta, CD4, CD2, CD56, CD7, CD8, CD3, CD16, CD13, CD64, CD14, HLA-DR, CD11B, CD15, CD123, CD117, CD33 , CD45, cKappa, cLambda, cIgG, cIgM, cIgA.
- Specimen Type, Preferred:
- Bone marrow
- Peripheral blood
- Specimen Type, Alternate:
- Fresh tissue
- Cerebral spinal fluid
- Fine needle aspirate
- Pleural fluid
- Ascites fluid
- Sample Quantity, Preferred:
- 2-3 mL bone marrow
- 5-10mL whole blood
- 0.5cmx0.5cmx0.25cm 1 piece of tissue
- 10-100mL of fresh fluid
- Sample Quantity, Minimum:
- 0.5mL bone marrow
- 1mL whole blood
- 100mg fresh tissue
- 3mL fresh fluid
- (minimum volume is dependent on cellularity)
- Transport Container:
- Green top (Sodium Heparin) tube
- Lavender top (EDTA) tube
- RPMI medium
- Sterile saline
- Transport Temperature:
- Bone marrow/Whole Blood: Room temperature
- Tissue/fluid: Refrigerated
- Collection Instructions:
- Please submit a copy of the CBC if available.
- If testing will occur in less than eight hours, specimens must be handled and transported in a manner to assist in the maintenance of the specimen between 18-25 degrees Celsius prior to testing.
- If testing is expected to occur in greater than eight hours, specimens must be handled and transported with cold packs to maintain specimens between 2 and 8 degrees Celsius prior to testing.
- In event of a dry tap (no aspirate for flow cytometry) submit 5-10mL of whole blood (Sodium Heparin) or a separate 10mm core biopsy in sterile RPMI.
- Specimen should be received within 24 hours of collection for optimal cell viability.
- Specimen Stability:
- Room temperature: 2 day(s)
- Refrigerated: 2 day(s)
- Frozen: Unacceptable
- Rejection Criteria:
- Clotted
- Frozen
- Lithium tube
- Hemolyzed
- Any form of fixatives
- Clinical Significance:
- Detects plasma cells neoplasm
- Limitations:
- Fresh Specimen
- Turnaround Time:
- 1 day(s)
- Reference Ranges:
- NA