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MITF

Alias/Synonym: Microphthalmia Transcription Factor

CPT Code: 88342

Includes:
Specimen Type, Preferred:
Formalin fixed paraffin embedded tissue
Specimen Type, Alternate:
Sample Quantity, Preferred:
1 block
2 slides
Sample Quantity, Minimum:
N/A
Transport Container:
Paraffin block
Unstained slides
Transport Temperature:
Room temperature
Collection Instructions:
Block and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Slides should be positively charged and cut at 3-4 microns for each stain/antibody ordered.
Specimen Stability:
N/A
Rejection Criteria:
No liquid specimens.
Uncharged slides
Clinical Significance:
MiTF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium, and also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1 and TRP2. MiTF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MiTF transcriptional activity. Multiple isoforms of MiTF exist, including MiTF-A, MiTF-B, MiTF-C, MiTF-H, and MiTF-M, which differ in the amino-terminal domain and in their expression patterns. The MITF-M isoform is restricted to the melanocyte cell lineage. Anti-MiTF, D5, recognizes a nuclear protein and is a useful aid for classification of primary and metastatic epithelioid malignant melanomas.
Limitations:
Turnaround Time:
1 day(s)
Reference Ranges:
N/A