IgvH Mutation Analysis
Alias/Synonym:
CPT Code: 81263
- Includes:
- Specimen Type, Preferred:
- Bone marrow aspirate
- Formalin fixed paraffin embedded tissue
- Fine Needle aspirate
- Specimen Type, Alternate:
- Peripheral blood
- Sample Quantity, Preferred:
- 2mL aspirate
- 10 mL peripheral blood
- 1 Block
- 20mL FNA
- Sample Quantity, Minimum:
- 0.5mL aspirate
- 5mL peripheral blood
- 5 unstained slides
- 10mL FNA
- Transport Container:
- Lavender top (EDTA) tube
- Green top (Sodium Heparin) tube
- Paraffin block
- Unstained slides
- Alcohol based fixative (e.g. Cytolyte) for FNA
- Transport Temperature:
- Refrigerated for tubes & FNA
- Room temperature for blocks
- Collection Instructions:
- If bone marrow is dry tap, submit 5-10mL peripheral blood.
- Specimen Stability:
- Room temperature: 2 day(s)
- Refrigerated: 3 day(s)
- Rejection Criteria:
- Room temperature or clotted for tubes
- Frozen
- Clinical Significance:
- Detects mutations from germline sequence of the variable region of the immunoglobulin heavy chain for Chronic Lymphocytic Leukemia (CLL) prognosis.
- Limitations:
- This test is designed to detect germLine sequences with ≥10% Bcell population.
- Turnaround Time:
- 7 - 10 day(s)
- Reference Ranges:
- Cutoff value % based on assay sensitivity