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ER/PR HER2, Reflex to HER2 FISH

Alias/Synonym: Estrogen Receptor/Progesterone Receptor, Her2Neu, PgR

CPT Code: 88360x3, 88377

Specimen Type, Preferred:
Formalin fixed paraffin embedded tissue
Specimen Type, Alternate:
Sample Quantity, Preferred:
1 block
2 slides
Sample Quantity, Minimum:
Transport Container:
Paraffin block
Unstained slides
Transport Temperature:
Room temperature
Collection Instructions:
Block and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Slides should be positively charged and cut at 3-4 microns for each stain/antibody ordered.
Specimen Stability:
Rejection Criteria:
No liquid specimens.
Uncharged slides
Clinical Significance:
Estrogen Receptor (ER) and Progesterone Receptor (PR) are measured in breast cancer specimens according to ASCO/CAP recommendations, for both prognostic and therapeutic purposes. Receptor expression is detected by binding of monoclonal rabbit anti-ER antibody clone 6F11 by Leica and monoclonal mouse anti-PR antibody clone 16 by Leica. ER and PR status is determined by the percentage of stained tumor cells, according to ASCO/CAP guidelines. HER2 (c-erbB-2) expression by IHC is measured in accordance with CAP/ASCO Guidelines for both prognostic and therapeutic purposes in breast and gastroesophageal carcinomas. HER2 expression level is detected by binding of a monoclonal rabbit anti-human HER2 antibody (Biocare clone EP3). HER2 expression level is determined by the percentage of tumor cells with complete or intense staining Her2 (FISH) detects HER2 gene amplification
Turnaround Time:
3-5 day(s)
Reference Ranges: