Alias/Synonym: Erythroid Precursors in Bone Marrow in the Determination of AML and MDS

CPT Code: 88342

Specimen Type, Preferred:
Formalin fixed paraffin embedded tissue
Specimen Type, Alternate:
Sample Quantity, Preferred:
1 block
2 slides
Sample Quantity, Minimum:
Transport Container:
Paraffin block
Unstained slides
Transport Temperature:
Room temperature
Collection Instructions:
Block and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Slides should be positively charged and cut at 3-4 microns for each stain/antibody ordered.
Specimen Stability:
Rejection Criteria:
No liquid specimens.
Uncharged slides
Clinical Significance:
The transferrin receptor (CD71) is essential for iron transport through the cell membrane. It is most highly expressed on actively proliferating cells, particularly placental syncytiotrophoblasts, myocytes, basal keratinocytes, hepatocytes, endocrine pancreas, spermatocytes, and erythroid precursors. The level of transferrin receptor expression is highest in early erythroid precursors through the intermediate normoblast phase, after which expression decreases through the reticulocyte phase.2,3,4 The maturation of erythrocytes results in loss of transferrin receptor expression, in concert with down-regulation of the machinery for hemoglobin synthesis. The high level of transferrin receptor within erythroid precursors makes it an excellent marker for evaluation of erythroid components within bone marrow. Anti-CD71 is a highly effective marker for highlighting erythroid precursors in bone marrow biopsy specimens and shows the following features: 1) distinct membranous and cytoplasmic staining pattern, which is easily recognized in bone marrow biopsy; 2) restriction to erythroid lineage within bone marrow biopsy specimens; 3) CD71 expression decreases with the maturation of erythrocytes, with the highest level seen in early forms and the lowest level in late normoblast stage, and most importantly; 4) mature erythrocytes do not express CD71, which facilitates bone marrow analyses. Anti-CD71 is useful in identifying erythroid precursors with very little interference from mature erythrocytes and also in the determination of erythroid leukemia, benign erythroid proliferative disorders, and myelodysplastic syndrome, although further studies are needed for making a definitive diagnosis of MDS.
Turnaround Time:
1 day(s)
Reference Ranges: