B Cell Clonality Assay
Alias/Synonym: Bcell
CPT Code: 81261
- Includes:
- Specimen Type, Preferred:
- Bone marrow aspirate
- Formalin fixed paraffin embedded tissue
- Specimen Type, Alternate:
- Peripheral blood
- Sample Quantity, Preferred:
- 2mL aspirate
- 10 mL peripheral blood
- 1 Block
- Sample Quantity, Minimum:
- 0.5mL aspirate
- 5mL peripheral blood
- 5 unstained slides
- Transport Container:
- Lavender top (EDTA) tube
- Green top (Sodium Heparin) tube
- Paraffin block
- Unstained slides
- Transport Temperature:
- Refrigerated for tubes
- Room temperature for blocks
- Collection Instructions:
- If bone marrow is dry tap, submit 5-10mL peripheral blood.
- Specimen Stability:
- Room temperature: 2 day(s)
- Refrigerated: 3 day(s)
- Rejection Criteria:
- Room temperature or clotted for tubes
- Frozen
- Clinical Significance:
- Detects monoclonal B-cell immunoglobulin gene rearrangements of IgH framework regions in lymphoproliferative disorders (LPDs).
- Limitations:
- This test is designed to detect B cell rearrangements involving IgH framework and joining regions.
- Turnaround Time:
- 7 - 10 day(s)
- Reference Ranges:
- Cutoff value % based on assay sensitivity